Sentence examples for despite different attempts from inspiring English sources

Only single TFIIAγ-like gene was isolated from both Cyperus rotundus and Zingiber officinale despite different attempts have been tried, including optimization of PCR amplification, recombination of up and down stream primers.

Despite eight different attempts at assessing and mapping health facilities data following the Haiti earthquake, the results of this study suggest that none provided a comprehensive assessment of emergency health services in a manner that corresponded with existing standards for humanitarian response.

Despite multiple attempts with different antibodies we were unable to immunostain for the ICC marker, c-kit.

It has not been possible to express the 7G8 domainomain despite repeated attempts with different domain boundaries [44].

Despite several attempts using different primer pairs we could not obtain a reproducible expression pattern of the AsCBF2 gene.

Out of the 120 accessions, no PCR product was obtained from eight C. melo wild relative accessions, despite several attempts using different PCR amplification conditions.

Despite several attempts, using different primer pairs we could not obtain a reproducible expression pattern of the AsCBF2 gene (data not shown).

Editorial compliance with the Declaration of Helsinki in rejecting research that is conducted unethically was difficult to ascertain because of a poor response rate despite multiple attempts using different modalities.

For CDS in Leucanthemum vulgare and FDS2 in Aster ageratoides, we were unable to isolate a full-length cDNA despite great attempts using different cDNA templates, primers, PCR programs and annealing temperatures.

However, despite several attempts in different systems we were unable to clearly dissociate the appearance of LC3 puncta from the release of cytochrome c following either Bax or PUMA expression (Supplementary Figure 3).

Despite several attempts using different primer pairs, we could not obtain reproductive expression patterns of 23 transcripts, thus they were shown as blank in Additional file 3. The qRT-PCR results confirmed that 85% of the abiotic stress-responsive genes (82 of the 97 detected uniscripts) were cold- or drought-inducible in A. mongolicus.