Despite being straightforward, this procedure is not widely used in gene and cell therapy for various reasons, including low transfection efficiency of primary cells, likelihood that plasmid fragments are randomly inserted into the gene, cytotoxicity associated with the use of DNA, and bacterial DNA sequences present in plasmid backbones (Maeder and Gersbach, 2016; Wang et al., 2016).
Despite many NTDs being straightforward to prevent or treat and progress being made to treat more people than ever before, they are still rarely prioritised in global health spending.
Despite appearing to be straightforward, the process of natural selection is frequently misunderstood by high school students (Demastes et al. 1995), undergraduates (Bishop and Anderson 1990), biology majors (Dagher and BouJaoude 1997), medical students (Brumby 1984), and science teachers (Nehm and Schonfeld 2007).
According to executive producer, Teemu Hämäläinen, the development process hasn't been straightforward, despite the familiarity of the genre.
But against a side as disciplined and obdurate as Stoke that was never likely to be straightforward, despite their woeful record at Stamford Bridge.
Keane insisted that his negotiations with Football Association of Ireland chief executive John Delaney in advance of accepting the role had been "straightforward" despite his previous trenchant criticism of the Irish official.
Otherwise his path through the potential complexities of their relationship is straightforward, and despite grief, heroism and armed incursion, without much surprise.
Despite this comment, the analyze is straightforward and carefully carried out.
Note, however, that such procedures are not widely used in practice despite the fact that they are straightforward and simple to implement.
Despite the rather large amount of data, the results are straightforward.
It's straightforward and the build quality is acceptable despite its appearance.
