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Using a cheese iron, a long skinny tool loosely resembling an apple-corer, to sample the cheese center for readiness, Mr. Skovron cut a narrow passage into the wheel, extracted a strip of cheese, sampled, pronounced it at a desired stage of ripeness, reinserted most of the sample back into the wheel, and smoothed over the rind.
Plugged females at the desired stage were sacrificed.
Drosophila embryos were collected at the desired stage, dechorionated in 50% bleach and rinsed thoroughly using distilled H2O.
Embryos were collected in egg laying agar plates and grown at 25 or 18°C until the desired stage.
At 11 hpf, embryos were heat-shocked at 39°C for 30 minutes and then returned to 28.5°C until the desired stage of development [21].
The injection sites were sealed with melted dental wax, and the animals were returned to the rearing room and allowed to develop to the desired stage.
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For expression analysis of different development stages, penultimate leaves were collected at the desired stages for analysis of the expression of OsHox32.
Microinjection of mRNA and siRNA into mouse preimplantation embryos were performed on a Leica DMI3000B microscope equipped with a Leica micromanipulator as previously described (Na and Zernicka-Goetz, 2006) at desired stages.
For immunostaining, embryos were collected at desired stages (9.5 10.5 dpc) and fixed in 4% PFA.
Embryos at desired stages were fixed with 4% paraformamide (PFA) overnight at room temperature (RT).
Fertile chicken eggs were purchased from Shiroyama Farm (Kanagawa, Japan) and incubated at 38.5 degrees to desired stages.
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