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After several unsuccessful experiments, we finally found that the desired sequential reaction could be realized smoothly in toluene (Table 1, entry 6).
The variable BsaI overhangs in the destination vector and in each of the modules' extremities were designed to allow seamless ligation of all inserts in the desired sequential order.
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In order to reduce the number of simulations needed to achieve the desired accuracy, sequential design methods can be employed.
Structures can easily be formed in silicone phantoms through sequential molding, i.e., by curing each desired feature sequentially.
The actual realization was carried out by acquiring the desired properties in sequential steps which is in detail.
The implementation of sequential simulation consists of reproducing the desired spatial properties through the sequential use of conditional distributions (Arpat 2005; Dimitrakopoulos and Luo 2004).
1-5 mg of purified DNA was treated with sodium bisulfite (10), then the desired sequences were amplified in sequential fragments using nested PCR.
RAGE (RNAi-assisted genome evolution) is a recently developed genome engineering method that can continuously improve a desired trait by allowing the sequential introduction of tractable reduction-of-function modifications to the genome [ 19].
Fig. 16 Top (a) and side (b) views of microfluidic EWOD chip highlighting hydrophilic patches designed for controlling deposition of materials on desired regions only, c illustration of sequential deposition and washing through control of droplet path by electrowetting.
In addition, recursive genome transfer and screening allows further enhancement in gene recombination and sequential addition of the desired traits.
Initial contacts were made through dialing those numbers in a sequential order until the desired number of controls was obtained.
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