Sentence examples for desired nucleotide from inspiring English sources

Six hybrid chimeraplasts of identical length were constructed with various lengths of target homology and strand location of the desired nucleotide change.

However, the strand placement of the desired nucleotide change and the length of nonhomologous sequences flanking target nucleotides played a crucial role in the efficiency of chimeraplast-mediated gene correction.

Sequencing confirmed the presence of the desired nucleotide substitutions.

Sequencing of double-stranded plasmid DNA by the Sanger method was used to confirm the desired nucleotide substitution.

The mutations were introduced into the plasmid pRPCaMDR1-GFP according to the manufacturer's instructions, and the desired nucleotide sequence alterations were confirmed by DNA sequencing of the ORF.

These two primer features have been exploited to allow the amplification of only the desired nucleotide sequences and not other (unwanted) very similar DNAs [39] [41].

The most significant impurities found in synthetic oligonucleotides are the (n-1) deletions that differ from the full-length product by lacking only one of the desired nucleotides.

The resulting mutants were sequenced to confirm the presence of the desired nucleotides changes and to rule out any undesired mutations introduced during the mutagenic procedure.

Subsequent action of galactose-1-phosphate uridylyltransferase would then give the desired sugar nucleotide (Fig. 1A).

Integrative 3'-overlapping recombinant oligonucleotides harbouring the desired single-nucleotide substitutions were extended in vitro as described by Storici et al [ 18].

To check if the nmt1 promoter and EGFP were joined correctly, we sequenced the connecting region; 2 out of 7 plasmids with the desired inserts had nucleotide substitutions within the connecting region (the primer region for PCR) (Table 2).