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But the chopped steak, ordered medium, was gray and dry, as was the rack of lamb, desired medium rare.
After sonication, the FBS suspension was diluted to the calculated volume with the desired medium (90%% of the total volume) and re-sonicated at 1.05 W for an additional 20 min.
Finally, cells were resuspended in the desired medium and plated.
The Collagenase IV was removed and replaced with the desired medium formulation.
PMNs were suspended in desired medium for downstream assays, and the purity/viability was typically >90% as evaluated by flow cytometry.
Cells (2000 cells per well) were seeded on a 96-well plate in 100 μL of desired medium and incubated for 24 h.
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These parameters give spectral tuning of the plasmon resonance to overlap the emission spectrum of the desired active medium.
After treatment with the desired products, medium was collected and stored at –20°C until prostacyclin was measured.
After 24 hours of incubation with the desired treatments, medium was collected and stored at –20°C until asymmetric dimethylarginine (ADMA), a major endogenous inhibitor of nitric oxide synthase (NOS), quantification.
The gelling agent was applied to the desired dispersion medium by pipette addition to a final concentration of either 5 or 10 mg/ml; the higher concentration corresponded to increased gel stability.
One of these new currencies, the Florin, became the most desired and respected medium of exchange in the Europe that made the Renaissance – the dollar of its day.
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CEO of Professional Science Editing for Scientists @ prosciediting.com