After filtration through a filter paper to remove fibroin fibers, the SS solution was concentrated until the desired concentration was achieved (approximately 7 wt%, measured by the BCA protein assay kit, Pierce, Rockford, IL, USA).
After filtration through a filter paper to remove fibroin fibers, silk sericin solution was concentrated until the desired concentration was achieved (approximately 7% w/v, measured by the BCA Protein Assay Reagent, Pierce, Rockford, IL, USA).
The dialyzed protein was concentrated in Micron columns (Millipore) and adjusted to the desired concentration.
The soluble POM2 scFv was lyophilized to concentrate and then resuspended in sterilized water to the desired protein concentration for subsequent experiments.
Purebred production, or line breeding, is used to concentrate desired genes for example, litter size or growth rate within a population of animals.
The selective removal water by the membrane separator has the desired effect of concentrating the organic pollutants resulting in higher conversion and mineralization rate.
With NSASC/NSAMC, the null positions can be selectively tracked one after another without affecting other null positions so that the interference signals can be rejected while the desired signal is concentrated by the array.
In one example, the overall recovery of the concentrate stream is maximized for a desired grade of the concentrate and a fixed total cell volume.
Optional: sweeten crust with fruit juice concentrate such as apple or white grape juice concentrate, if desired.
All 10 supercars have automatic transmissions, some paddle-shifted and some floor-shifted, and all can be left in the fully automatic mode, if desired, so students can concentrate on the driving.
