Sentence examples for desired alterations from inspiring English sources
As with earlier ZFN-editing experiments [ 6], the desired alterations were probably enriched within the bulk parasite population by the inclusion of silent mutations in the donor sequence that protected both the donor plasmid and edited genomic locus from cleavage.
The flexibility and ease of use of CRISPR has facilitated the rapid generation of almost any desired alteration with greater efficiencies and lower costs than previous approaches.
After the desired alteration had been made, the two-ARS plasmid was reconstructed.
In mutant screens, typically hundreds or thousands of mutants are generated and screened for a desired alteration in a specific phenotype.
ZFN-driven genome editing has allowed the rapid development of cell lines and animal models harboring almost any desired alteration to the genome (Hockemeyer et al., 2009; Holt et al., 2010; Moehle et al., 2007; Urnov et al., 2005).
A system is built to create such a desired alteration for the output.
Alteration of pain or taste perception often was taught in a few minutes, by suggesting that the patient imagine being in a relaxing place, followed by a suggestion that yielded the desired change in perception.
In our model experiments, the percentages of mutants containing the desired nucleotide alterations at all sites ranged from 60-80% after four rounds of mutagenesis had been performed.
Our study provides evidence for an embryonic origin of putative mammary stem/progenitor cells and informs the use of specific cytokeratins as markers of mammary epithelial differentiation, as well as the use of their promoters to direct desired genetic alterations.
A promising alternative to gene-addition is in-situ replacement of disease-causing gene mutation by homologous recombination (HR) between the endogenous genomic sequence and an exogenous DNA template harbouring desired sequence alterations to correct the genetic defect.
Nevertheless, low 20% ORF values obtained from experiments using anionic surfactants indicated that the desired wettability alteration is not always achieved.
