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Screening designs were performed to identify critical process parameters (CPPs).
Subsequently other studies with similar designs were performed.
Magnetic and conceptual designs were performed by SOLEIL, and the technical realization was carried out together with different manufacturers.
Single-factor experiments and orthogonal designs were performed to determine the effects of extraction conditions on polysaccharide yields.
Preliminary studies using factorial designs were performed to determine which operational parameters should be included in the optimization stage.
Full factorial experimental designs were performed to evaluate the above treatment technique and to optimize the processes.
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Risk analysis and D-optimal statistical design were performed.
Conception and design were performed by Kang-Ju Kim. Kang-Ju Kim
Study concept and design were performed by JDW.
DNA sequence alignments and primer design were performed using Primer Premier 5.0 (PREMIER Biosoft International, Palo Alto, California, USA).
SNP selection and assay design were performed according to previously described procedures [ 32].
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