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Together, our findings strongly suggest that the strategy herein developed combining heterologous expression using a cloning vector carrying the pAMβ1 replication origin and experimental designs optimization can be generalized for recombinant proteins production in Bacillus.
In conclusion, thanks to heterologous expression of the phytase gene from B. subtilis US417 in B. subtilis 168 using the new efficient expression system developed and applying experimental designs optimization, we succeeded to reach maximum phytase yield of 47 U ml-1 which represents one of the highest phytase activity achieved so far in Bacillus.
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Table 1 D-optimal design optimization of J. curcas seed oil hydrolysis and response for FFA%.
Hence, for large designs, optimizations must be performed.
Optimized design: Optimization following a specific algorithm (I-optimal, D-optimal, A-optimal).
For example, Boeing made available its Multidisciplinary Design Optimization code, WingMOD, which optimizes the aircraft planform for a given mission.
Artist, architectural designer, and urbanist Wendy W Fok conceptualizes structures with the use of digital design, optimization, and fabrication to challenge past theories, and create alternative and original designs for the future.
Engineering Design Optimization.
Philippe M. Manne, "Design Optimization of Composite Plates".
As products become components of broader systems, the opportunities for design optimization multiply.
This study describes the design, optimization, characterization and image generation using a 3D Light PAD.
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