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We further showed, when the models were activated by transient signal, memory (total time taken by the cascade output to reach its unstimulated level after removal of signal) of a cascade was determined by the specific designs of interaction among its kinases and phosphatases.
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The three layer mitogen activated protein kinase (MAPK) signaling cascade exhibits different designs of interactions between its kinases and phosphatases.
Here, we built computational models of four experimentally observed designs of interactions among the kinases and phosphatases of the MAPK cascade and compared the robustness and signal response behavior associated with each of the designs.
We found that irrespective of the parametric differences in K1 and K2 type models, the MAPK cascade's robustness and signal response behaviour are pivotally shaped by the designs of interactions among its kinases and phosphatases.
To elucidate the generalized nature of behaviors emerging pivotally from the different designs of interactions among kinases and phosphatase in the MAPK cascade, we carried out our studies adopting both steady state kinetics (K1) and elementary mass action kinetics (K2).
This project was assigned to a team of 4 members with background in industrial design, design of interaction, visualizations, systems design and engineering.
Focus areas include understanding the theory and mechanics of both human and computer perception and cognition, the design of interaction architectures such as teleoperation and human supervisory control, and how to conduct principled tests and experiments.
The results also suggest that Galinter has the potential of assisting in the design of interaction inhibitors.
Design of interaction entropy graphs was accomplished using the Orange software [ 75].
Finally the MAPK cascade exhibiting more complex design of interaction such as M4 is such that MKKK and MKK are dephosphorylated by Phos1 whereas MKK and MK are dephosphorylated by Phos2 [ 14].
Based on our study it could be argued that differential designs of kinases-phosphatases interactions may have evolved to satisfy specific cellular requirements, such that need based specificity in signaling could be achieved in a non-trivial manner adopting a certain design of interaction among the phosphatases of the system.
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