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When designing a guide sequence for genome targeting, the specificity in terms of off-target potential is crucial.
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When approached by the museum to design a guide, Mr. Otterness walked through the galleries sketching and making notes.
To experimentally implement the CRISPR/Cas system for a specific goal one needs to design a "guide" sequence, the gRNA, that is typically 20 nts in length and complementary to the intended target [ 13- 16].
Matt is starting his own company, HelloCity, and designing a city guide app.
There are several factors to consider when designing a cutting guide for a surgery.
We considered designing a screening guide, adapted to the conditions of the emergency services, to identify AE.
So, T editors scoured the Internet and designed a foolproof guide to shopping the best discount sites.
Based on the surgical simulation results, we designed a surgical guide using the Mimics software.
The simulation allowed us to design a surgical guide for genioplasty, which was then computer-rendered and 3D-printed.
Preoperatively, the authors designed a surgical guide using 3-dimensional printing and virtual surgery.
These conceptual objectives were used to design a Discussion Guide for the Internet Focus Groups (IFGs).
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