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The resulting performance usage scenarios are provided as a tool to help stove designers, program implementers, policy makers, and other stakeholders consider the most appropriate technology and behavior change pathways for achieving maximal impact.
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A pants count of the designers' programs tells the story: Donna Karan's DKNY, 1 pair out of 27 outfits; Derek Lam, 9 of 33; Tommy Hilfiger, 10 out of 43; D.V.F., 16 out of 48 — a neat one-third.
To create the seats, the designers programmed a custom 3D printer to produce ribbons of cement (in a mixture reinforced with nylon fibers) in a succession of thin layers.
Primers were designed with Beacon Designer program (Premier Biosoft, Palo Alto, CA) as it follows: TXNIP SENSE 5'-TCA Tgg TgA Tgt TCA AgA AgA TC-3'; ANTISENSE 5'-ACT TCA CAC CTC CAC TAT C-3'; TRX SENSE 5'-CAg ggg AAT gAA AgA AAg g-3'; ANTISENSE 5'-CAA ggT gAA gCA gAT Cg-3'; β-ACTIN SENSE 5'-TTT gAA TgA TgA gCC TTT gTg-3'; ANTISENSE 5'-TCA gTg TAC Agg TAA gCC CT-3'.
All primers (Additional file 2) were designed using the Beacon Designer program (Premier Biosoft, Palo Alto, CA).
Gene-specific primers for the identified genes were designed using the Beacon designer program.
Expression analysis and normalisation was performed in R using the RMA package, following a previously established pipeline [ 53] Three artificial miRNAs targeting SQN were designed using the Web MicroRNA designer program; http://wmd3.weigelworld.org/cgi-bin/webapp.cgi[ 54] and cloned into the pJet2.1 expression vector.
Since 2002, Lutz & Patmos had a guest designer program, with participants collaborating on unique sweaters.
To design biorefinery processes producing bioethanol from lignocellulosic biomass with dilute acid pretreatment, biorefinery processes were simulated using the SuperPro Designer program.
Last year, designers who participated in a Vogue and Council of Fashion Designer program were asked to create a one-of-a-kind item inspired by Google in some way that reflected Google's aura.
The ccdB cassette with the appropriate ends was optimized for expression in E.coli using the Gene Designer program (DNA 2.0 Inc).
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