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Specific primers for each gene were designed using Beacon Designer version 7.0 (Premier Biosoft, Palo Alto, CA) (Additional file 12).
The primers were designed with Beacon Designer, version 7.7 (Premier Biosoft International, Ltd., Palo Alto, CA, USA).
Primers were designed using Beacon Designer, version 7.2, selecting for a primer annealing temperature of 55 °C and amplicon length of <100 bp.
The primers for PCR were designed using software Beacon Designer Version 7.9 (Premier Biosoft International, Palo Alto, CA, USA).
The design, mass balance, and energy balance simulations were performed using SuperPro Designer (Version 8.5003, 2012).
Moreover, a custom-made item is often cheaper than a designer version.
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The difference is that the designer versions are marginally sexier, Ms. Albright said, cut low in the vamp to show toe cleavage.
To judge by the videos the industry cranks out, misbehavior in this genre utopia doesn't get much racier than beer-and-bonfire jams attended by models decked out in designer versions of working-class attire.
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