Sentence examples for designed with use of from inspiring English sources
PCR amplification was 94°C for 3 min, 35 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec, then 72°C for 3 min. To increase the specificity of gene amplification, primer sets were designed with use of Vector NTI (v9.0) with the 3'UTR sequence for each OsADF gene, except for OsADF7, OsADDF8a and OsADF8b, whose primers matched the coding region.
Primers were designed with use of Primer 3 [ 18, 19].
MET shRNA sequence 5′-AGUCCGAGAUGAAUGUGAAtt-3′ has been designed with use of available algorithm from Ambion http://www.ambion.com/techlib/misc/siRNA finder.html.html
Bisulphite-converted DNA samples were pyrosequenced using specific sequencing primers designed with the use of the PyroQ assay design software (Pyromark MD, Qiagen), and assay were performed on a Pyromark MD pyrosequencer using standard protocols and controls.
Custom 16q24 region-specific high-resolution 44K microarrays were designed with the use of eArray (Agilent Technologies) and used for CNV screening in 14 mutation-negative patients with ACD/MPV.
The item content and layout of the RetTSQ was designed with the use of semi-structured interviews with patients with diabetic retinopathy of varying degrees of severity following various treatments.
