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Each student is receiving a Samsung flip-phone in a package specially designed with the program's logo.
They were designed with the program Primer3 [ 21] and synthesised by Microsynth.
Briefly, 20-mer oligonucleotides were designed with the program Primer 3 [ 49].
Primers (Table 1) were designed with the program PCR Suite [ 30] and modified afterwards, if needed.
Oligonucleotide primers were designed with the program eprimer3 from the EMBOSS program package (version 5.0.0, http://emboss.sourceforge.net/).net/
Sense and antisense sequence of oligonucleotides for S100 andand 9, SPRR3, NICE-3 and -4 genes were designed with the program Primerquest [ 38].
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Forward and reverse primers for β-actin, acyl-CoA oxidase (AOX), lipocalin 2 (Ngal), kidney injury molecule 1 (KIM1), heme oxygenase 1 (Ho1), stearoyl element binding protein-1c (SREBP-1c), fatty acid synthase (FASN), and acetyl-CoA carboxylase 1α (ACC1α) were designed with the FastPCR program (R. Kalendar, Institute of Biotechnology, Helsinki, Finland).
PMMoV sequences corresponding to two different regions of the PMMoV genome were obtained by RT-PCR amplification using the SuperScript One-Step RT-PCR System (Invitrogen Life Technologies) and primers that had been designed with the SVARAP program [11].
tRNAs with unusual structure, such as those lacking an arm, were searched also using specific patterns designed with the PatSearch program (Pesole et al. 2000).
Moreover, tRNAs with unusual structure, such as those lacking an arm, were searched using specific patterns designed with the PatSearch program (Pesole et al. 2000).
Primers for the TRPS1, EIF3S3 and MYC genes were designed with the Primer3 program (available at http://www-genome.wi.mit.edu/cgi-bin/primer3.www.cgi).
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