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The modifications to the vector were again confirmed by sequencing, and we designated the designed vector as pAAV-LIC.
To overcome this problem, barstar gene under its own minimal cytomegalovirus promoter was used in designed vector.
Second, the deletion of episomal SV40 origin and neomycin selection gene region of pcDNA 3.1 diminished the size of the designed vector by more than 2 kb, which would greatly increase the capability of the designed vector to clone large PCR products.
LIC requires a specially designed vector containing a long stretch of sequence that is missing any one of the four nucleotides.
The designed vector pOmni (Fig. 1) was constructed on the basis of pcDNA 3.1 through four times of PCR-mediated mutation, in which one is deletion and three are insertions.
The HEK 293T cells were transfected with the designed vector, and the 4D5 scFv-barnase-His5 fusion protein was identified by anti-barnase antibodies in cell culture medium and after purification from cell lysates using metal-affinity chromatography.
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The designed vectors exhibit considerably higher cell biocompatibility and better resistance against bovine serum albumin adsorption than PEI5k.
We have encountered artificial chromosomes generated from pTACs in transformed T. cruzi epimastigotes for every version of the designed vectors.
CP designed vectors and performed embryonic stem cell manipulation.
That is, design vector x1 is superior to design vector x2 when (I_{text{FR}} left( {{varvec{x}}_{1} } right) = 1) and (I_{text{FR}} left( {{varvec{x}}_{2} } right) < 1).
We will explore how to design vector quantizers and how these quantizers can be used for compression.
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