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Primers designed upstream of the initiation codon amplified the insertion.
For those cases where the N-terminus included important structural elements that were resolved in the 3D template, the truncation site was designed upstream of this structural element.
It is found that the designed upstream injection of baseline case effectively reduces the heat transfer to body surface compared with the no-jet case.
In particular, primers were designed upstream and downstream the chosen diagnosis site in a well conserved region for both Bos taurus and Bubalus bubalis.
Sliding in a dam foundation along potential sliding paths is generally caused by two kinds of external factors: one is the overloading of the designed upstream hydrostatic load due to flooding; and the other is the gradual degradation of the shear strength of joints due to seepage, deformation, damage, and geochemical reactions between water and joint surface minerals.
The sense primer was designed upstream of the mapped genomic location, and the antisense primer was designed based on the 3' end sequence of each 3' EST.
The forward primer (SF1, Table S1) was designed upstream of the bovine open reading frame (ORF) of SPRN and the reverse primer (SR1, Table S1) was designed downstream of the sheep MGT1 sequence using the software Primerselect™ (Figure 1).
3C primers were designed upstream and downstream of the core promoter site (P).
Two 90 nt oligonucleotides were designed upstream and downstream the HIS3 insertion site.
Specific primers for TaSK2 sequences were designed upstream and downstream of this restriction site (Table 2).
The primers were designed upstream and downstream of SSR sites with sequence length ranging from 18 to 22 bp.
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