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Like the 42 bp synthetic DNA controls, this 141 bp fragment was also designed to be nested within the 203 bp region routinely amplified for MALDI-TOF MS genotyping to minimize the possibility of false positives due to PCR amplification of small amounts of any contaminating K211 cDNA.
Samples were spotted in nanoliter amounts onto a matrix-arrayed silicon chip with 384 elements and analyzed with the manufacturer's MassARRAY compact system and software (Sequenom, Inc .. Synthetic DNA controls (42 bp) were designed to be nested within the 226 bp hME PCR product to minimize the potential amplification of any cross-contamination between synthetic K211 alleles and animal samples.
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The use of a multilevel model will be applied for a number of reasons: patient PDU is hypothesized to be dependent on the prescription policy of the physician and thus to be nested within DSCUs, the longitudinal design and cluster randomization, and the replacement of drop-outs.
Megawidgets can be nested within other megawidgets to support more complex interactions.
Elements can be nested within each other.
Due to the sampling design, Sites are nested within Regions, denoted as Site(R), and M. capitata colonies are nested within Sites, denoted as Colony(S R)).
Such a design is also encountered in individually randomized trials in which clustering is imposed by the intervention design and is nested within groups, such as when subjects are assigned to two treatment arms for which the intervention is delivered by several physicians, each participating in only one arm of the study [ 8, 9].
Since in this design patients are nested within therapists, these nested data will also be examined in a multilevel analysis, controlling for therapist variation.
Additionally, a pre-post intervention two-sample design is nested within the intervention arm to assess the incremental effectiveness of CIS + FI compared to CIS without FI on study outcomes.
A pre-post intervention two-sample design is nested within the CIS arm to assess the incremental effectiveness of the CIS plus financial incentives (CIS + FI) compared to the CIS without FI on study outcomes.
A case-control design was nested within a prospective cohort of healthy pregnant women.
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