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Specific primers have been designed to amplify complementary DNA fragment of LeGAPDH (143 bp), LeACO1 (240 bp), LeACS1A (169 bp), and LeACS2 (148 bp) using polymerase chain reaction.
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A multiplex PCR reaction was designed to amplify norovirus, astrovirus, and sapovirus complementary DNA (cDNA) present in the reverse transcription reactions described above.
For the moricin family, three overlapping and complementary primers were designed to amplify the chosen moricin paralogs (Table S1).
Separate primers were designed to amplify sense and antisense strands, because after bisulfite conversion the two strands were not precisely complementary, with additional primers designed to perform nested PCR, using Methyl Primer Express software v. 1.0 (Applied Biosystems, USA).
DNA was amplified by PCR using primer pairs designed to amplify TFPI2 promoter or TET1 promoter.
Amplifiers are designed to amplify signals.
Gene-specific primers were designed to amplify the Exp2 gene.
Fifteen primers were designed to amplify specific altered genes.
Specific primers were designed to amplify individual isoform transcripts.
Also, nested PCR was performed with primers designed to amplify species of the Phylum Firmicutes.
The primer pair was designed to amplify a region distinguishing the two divergent mitochondrial lineages.
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