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After that, in order to compare the effects of ASC-CM and each cytokine, another experiment group was designed: the culture medium was also changed with DMEM/F12 with EGF, PDGF-AA, VEGF or bFGF (at their optimal concentration).
After that, to compare the effects of ASC-CM and each cytokine, another experiment group was designed: the culture medium in the lower chamber was changed with DMEM/F12 with EGF, PDGF-AA, VEGF, or bFGF (at their optimal concentration).
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A closed, recirculating seawater system with a denitrification process was designed for the culture of black tiger shrimp broodstock.
Genet lines were cultured on portable mesh fabric (SEFAR NITEX, Switzerland) anemone substratum in a 30 litre apparatus designed for the culture of benthic marine invertebrates [43].
To design the patterning culture system using these cells, cell-recognizable polymers should be useful to regulate not only the hepatocytes, but also the nonparenchymal cells.
The amount of delivered oxygen is a significant factor in designing the cell culture bioreactors.
SG performed and helped design the cell culture, qPCR, microarray, hypo-osmotic stress and gene disruption experiments.
DLC designed the study, supervised cell culture, and drafted the manuscript.
MAJZ conceived of and designed the study, performed cell culture, data analysis and interpretation, bioinformatic analysis, and wrote the manuscript.
ST maintained all of the cell cultures, designed the siRNA experiment, ran qRT-PCR and western blots, performed the in vivo study, was involved in the design of the study and critically revised the manuscript.
This research contributes to the existing literatures and the findings will be useful for top management to design the corporate cultures strategically for better performance.
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