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Various sequence optimization algorithms were compared and two resulting designed sequences were experimentally evaluated.
The designed sequences do not have significant similarity to those of any natural proteins.
Analysis of the designed sequences revealed features that precisely define the rotation of the helices.
In addition, a number of single point mutants and three designed sequences are examined.
This robustness holds over a surprisingly wide range of folding rates for our designed sequences.
This percentage increases to 77% if we consider the three-dimensional models from the top 10 designed sequences.
Recent advances in protein structure prediction have provided potentials for an automated assessment of the designed sequences via folding simulations.
The simulated force-extension profiles for both random and designed sequences have attained quantitative agreements with the experimental data.
They sat astonished as we kids performed a couple of well-rehearsed, fully costumed and imaginatively designed sequences from the masterwork we were all living upstairs nightly.
"Alfred claimed he was a programmer who designed sequences where kidnapped boys were experimented on to become psychic spies and he said he was part of these psychic manipulations," said Garetano.
The designed sequences are available at http://biology.polytechnique.fr/biocomputing/sequences.fr/biocomputing/sequences
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