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Overall DSB repair was determined by counting γH2AX foci remaining 24 after irradiation, while NHEJ activity was monitored by using a specially designed repair construct stably integrated into the genome.
In human cell lines, precise deletions have been achieved using an appropriately designed repair ssDNA (Chen et al., 2011).
We successfully designed repair templates targeting 17 unique sequences in 11 different genes using genome sequence information available in WormBase, suggesting that, even when relying on microhomology, polymorphisms that could interfere with HDR are not an issue at least in the common C. elegans lab strain Bristol (N2).
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The design equations from the design model and the resulting values for the designed repaired specimen are listed in the equations from Eqs. (1) to (9).
When designing repair mortars, it is important to consider all its components such as the binder and the aggregates since they have a great influence on the mortar's final properties.
We conclude that, for precise edits, it is best to design repair templates with homology arms that extend as close to the cut site(s) as possible at least on one side of the cut.
This chapter illustrates how the damage-tolerant design approach discussed in can be used to design repairs to multisite damage in civil transport fuselage lap joints.
Pennsylvania released $700,000 to design repairs on the remaining towers and plan development of the new park facilities in June 2005.
Only thing is to do is open the standardized procedure for that component and follow the step by step activity to repair that component with optimized repair processes designed by repair designer.
The $25 million project is designed to repair and expand the ring road around Peshawar, the capital of the province, they said.
The reforms – designed to repair Fifa's image and guard against future misdemeanours – separate political power from the day-to-day running of the organisation.
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