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We present an effective method to assemble the PSs in specified regions by hydrophiling the substrate in designed regions for the nanophotonics with nanostructures in well-definite regions.
Target capture of custom designed regions and exome sequencing has allowed identification of causal variants in several Mendelian disorders [ 7], variants associated with complex diseases [ 8], and recurrently mutated cancer genes [ 9].
However, a maximum of 5 Mb of custom designed regions can be enriched with current protocols, which is little compared to for example 24 Mb for SureSelect hybrid capture.
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It is designed for regions that include many ethnic, religious, linguistic and cultural groups ― for regions like Rojava, which has Kurds, Arabs, Turkmen, Assyrians, Armenians, Sunni Muslims, atheists, Christians and many more.
All primers were designed in regions free of known SNPs.
RFLP markers were designed in regions with LOH.
Strain discrimination was not expected, since probes were designed from regions conserved within viral families.
In addition, nine probes were designed in regions that showed no evidence of DNaseI hypersensitivity.
Degenerate primers (TcNPR1dg-5', TATTGTCAARTCTRATGAARAAYCGTTTCCCKAGTTCCACRAAYCGTTTCCCKAGTTCCAC) were designed to regions highly conserved among all three sequences.
Second, for each next sequence oligonucleotides were only designed for regions that were not probed by previously designed oligonucleotides.
Degenerate primers were designed to regions conserved in these Caenorhabditis species and in Pristionchus pacificus (see Additional File 2).
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