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Primers were designed on each side of the putative gap.
We were able to obtain the full-length open reading frame (ORF) encoding the D1 receptor, The primers designed on each putative start (ATG) and stop (TAG) codons amplified the full-length ORF.
The array also displays oligos designed on each wBm gene model.
As an alternative, primers were designed on each 5′- and 3′-ends of the contigs and attempts to amplify every combination possible were unsuccessful.
To further characterize proteins binding on each FT, we performed electromobility shift assays (EMSA) using 25 nt long oligonucleotides designed on each of the six FTs that were incubated with either thyroid (PC Cl3) or non-thyroid (HeLa) protein extracts.
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Six flaps were designed on the dorsum of each dog.
For each gene, specific primer pairs were designed on the flanking regions of each MDP, isolated by aligning the UTR on the cDNA sequence, in order to define unique target elements for each polyphenolic biosynthetic gene.
PCR primers were designed on conserved flanking regions for each fragment, based on the genomic sequences and tested on all taxa (Table 1).
To my squealing girlish delight, Ms. Daugherty informed me that my pillow could be custom-made with both designs, one on each side, in my choice of colors, for the same price as the pre-made pillows in the store: $98.
For each siRNA, sense and antisense templates were designed based on each target sequence and partial T7 promoter sequence.
Four 60-mer oligonucleotide probes were designed for each gene on the microarray slide.
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