Specific primers for qRT-PCR are listed in Additional file 12. Primers were designed across neighboring introns using NCBI Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi).
Chicken and turkey genome conservation was determined by performing PCR amplification with forward and reverse primers designed on 13 neighboring short read turkey contigs aligning up to 0.5 kb apart on the chicken genome.
To screen for the 8 microbes indicated above, two multiplex PCR assays (MicroSHPT@5leX and MicroSHPT@3leX) were designed such that neighboring amplicons differed by 50 to 100 bps in size, helping to resolve PCR fragments into visible bands in agarose gel electrophoresis.
Recently, new fracturing designs in neighboring lateral wells have been introduced to enhance the production of trapped hydrocarbons, in particular in low-permeable shale reservoirs.
By objecting to a missile defense system that will never harm a human being, Russia's leaders are telegraphing to the world that they have expansive designs on neighboring countries and that they will expend whatever political capital it takes to keep them vulnerable.
On a larger scale, the rebels exploited differences among their white opponents--including Yucatan's inter-city rivalries--and took advantage of geopolitical events that distracted the attention of the central government: the Mexican-American War; Napoleon III's invasion of Mexico; and Mexican designs on neighboring British Honduras, now Belize.
Unlike the classical variational method to design the neighboring optimal control (NOC) by minimizing the second variation of cost functional, this paper, from a geometric point of view, presents a parametric approach to establish the NOC for the problem of fixed-time multi-burn orbital transfers.
The bending zone adaptable to the curved surface was designed between the neighboring PK structures.
Under Sargsyan's rule, Armenia bought weapons from Moscow and joined the Eurasian Economic Union, a Russian-backed economic bloc designed to deter neighboring countries from trading with Europe.
In the first step, a distributed cooperative guidance law is designed using local neighboring information for multiple missiles to achieve consensus on range-to-go and leading angle.
