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The idea is to flood the yeast with various designed mutations and see how they react.
Combining computationally and rationally designed mutations produced unique designs with heterodimer purities greater than 90%.
The thermostability of mesophilic xylanases has been increased considerably by designed mutations.
Sequences with designed mutations can be generated in this way to express altered protein sequences for structure function relationship studies.
Various synthetic peptides incorporating the designed mutations were produced and their helical content estimated by circular dichroism.
Moreover, temperature- and pH-activity profiles revealed that the designed mutations did not affect the catalytic activity of the enzyme.
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Saika, H. et al. Application of gene targeting to designed mutation breeding of high-tryptophan rice.
In this framework, the prior distribution undergoes an evolutionary process based on the designed mutation and crossover operators of GA.
The learning strategy for each swarm is the well-known comprehensive learning method with a newly designed mutation operator.
A combination of our best designed mutation, N71R, with another mutation, A85K, yielded a Raf mutant with a 100-fold improvement in affinity towards RasGDP.
The proposed method is able to outperform existing human designed mutation operators with statistical significance in most cases, with competitive results observed for the rest.
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