In batch fermentation, using the designed medium MW1 and MW2, the plasmid DNA yield was 60±5.0 and 30±4.0 mg/l, respectively.
The expanded MSCs in the designed medium preserved differentiation potentials into three mesenchymal lineages in vitro, including chondrocytes, adipocytes and osteoblasts.
This paper presents the simulation results of electric field distribution for optimally designed medium voltage composite insulators under polluted conditions with and without water droplets.
The maximum enzyme activity experimentally obtained using the ANN GA designed medium was 6.75 ± 0.09 U/ml which was in good agreement with the predicted value.
Influences of intercritical annealing temperature and duration on the microstructures and tensile properties of a newly designed medium Mn steel, Fe-7 wt% Mn-0.3 wt% C-2 wt% Al, have been studied and discussed in this paper.
This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC.
Effects of pH at uncontrolled-pH (pHUC = 6.0) and controlled-pH (pHC = 6.0) operations, and oxygen transfer at the air inlet rate of 0.5 vvm and agitation rates of 300, 500 and 700 min−1, were investigated in 3.0 dm3 bioreactor system with 1.65 dm3 working volume in the designed medium.
The resulting exocrine cells were divided into 2 groups: the first was cultured in a designed medium to allow differentiation into beta-like cells and the second was cryopreserved before the differentiation process at −196°C for 8 weeks before culture in the same medium.
Optimization of major physico-chemical parameters affecting enzyme production; including medium composition, pH, cultivation time and temperature revealed that maximum enzyme production was obtained in a self designed medium (pH 6.0) containing 1% Dextran 5000 Da, after 24 h culture incubation at 37 °C.
aU/mL bActivity peak of each enzyme produced using the designed medium.
cMeasured activity ratios of laccase/LiP produced using the designed medium.
