The activities measured are shown in Figure 2 and in Additional file 2, which also show the activities of variants designed in two subsequent design cycles.
Therefore a further regime was designed in which, subsequent to initial hydrolysis, phased additions were made at times where the material was deemed to have digested sufficiently.
cDNA integrity, as well as absence of genomic DNA contamination, was checked by GAPDH amplification with primers designed in two subsequent exons, thus generating different sizes for genomic DNA versus cDNA amplification products.
As they progress through their studies, there are opportunities to design experiments in subsequent courses.
Furthermore, as planning for each study region was completed, a formal "lessons learned" evaluation was conducted that informed process design in subsequent study regions.
One implementer did intentionally change the initial room design in subsequent classrooms.
Implementing a more comprehensive experimental design in subsequent studies is therefore essential to minimizing the thickness and positioning errors as well as to increasing the number of measurements for each pathology category.
We note, however, that variability in factors such as attention and the emotional valence of stimuli are fundamental to the study design in subsequent memory paradigms, affecting the probability of successfully encoding each item and thus enabling comparison of successful with unsuccessful encoding.
Based on the sequence of this amplicon, additional target-specific PCR primers were designed for use in subsequent rounds of 3′- and 5′-extension using DNA walking technique to eventually obtain a C. bombicola genomic sequence containing an open-reading-frame putatively identified as a glucosyltransferase (gtf-1).
The study's lead author, Barry Krakow, medical director of Maimonides Sleep Arts and Sciences in Albuquerque, designed a subsequent study to better understand insomniacs without obvious breathing issues — the 19percentt in his chart review study.
