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In view of this, an improved constraint-handling method is proposed which was designed in alignment with the constitutional mechanism of the proposed algorithm.
The organization has created monthly "Music-Versity" workshops, designed in alignment with standard for career and college readiness.
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The alignment designed in 1928 only remained intact eight years into 1936, when it was extended northward to its current terminus at the New York state line near Bear Lake, Pennsylvania and Niobe, New York in 1936.
The primers were designed in silico by sequence alignments based on available DNA sequence data.
Degenerate PCR primers targeting sxtT and sxtH used in this study (DioxF 5' CCNGARTGGGAYGAYCCNAAYTA 3' DioxR 5' ATRTCYTGYTCDATNGTYTCRTC 3') were designed in silico from sequence alignments produced using ClustalX [45].
The same pool also tested positive with primers (5´-CGTRGCAGCCACYTCATTAG-3´ and 5´-GTGTCGGCYGCSTTTGTTCC-3´) designed in this study from the alignment of the 13 sequences of TOSV retrieved from GenBank (accession nos. are shown in the Figure).
Small RNA sequencing tags were aligned against miRBase v15 pre-miRNA hairpins using miRNA-MATE, an open source alignment tool designed in our laboratory specifically for colour-space miRNA analysis (http://grimmond.imb.uq.edu.au/miRNA-MATE/; manuscript in preparation).
18S rRNA was cloned using primers designed in the program Primer3 [21] based on alignment of 18S rRNA from the gastropod Bursa rana (X94269.1) and the bivalve Nucula sulcata (AF207642.1) (Table 2).
From this alignment primers were designed in conserved regions using Primer3Plus [ 89].
The single closed fringe pattern that occurs in two superposed grating marks applied in the previously designed moiré alignment scheme based on dual-grating for lithography is processed and analyzed using a frequency domain method based on two-dimensional (2-D) analytic wavelet transform (AWT) and 2-D wavelet ridge algorithm.
For the nested PCR, new inner primers were designed from alignment of sequences circulating in Sweden and sequences from the GenBank database.
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