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According to the sequences of the CDR3 boundaries, we designed forward primers (shown in Figure 5), which were designated as V β20-1, -2, and -3, to perform the clonotypic PCR analysis for detecting the TCRs, the CDR3 sequences of which were identical to those cloned from the TCRV β20 gene.
A specially designed forward primer containing extended polyA20/5Sp9 facilitated gel electrophoresis purification of ssDNA after PCR amplification.
We designed forward and reverse primers so that they were positioned further away from the primer set contained in the Identifiler Kit.
A rationally designed forward primer, conjugated with a peroxidase DNAzyme complementary sequence at its 5′-end, is used for PCR amplification of target DNA.
Two oligonucleotide primers were designed, forward (5′-GGGAATTCCATATGGCCCAGGGCCAAGAATTCCACT-3′) and reverse (5′-CCCAAGCTTGGGTCAGAGCTTGTAGAATTTCTG-3′), to introduce the Nde I and Hin d III restriction endonucleases (Takara Bio Inc., Otsu, Shiga, Japan), respectively.
Our newly designed forward primer was compared to Holmes' forward primer and the results are shown in Tables 1 and 2. As the tables illustrate, with zero mismatches, pmoC374 is able to target three out of the seven available sequences from Verrucomicrobia.
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The Beacon Designer software was used to design forward and reverse primer and probe sequences for molecular beacon applications and were synthesized by Sigma Genosys Ltd ,Cambridge, UK.
For efficient non-uniform deblurring, it is crucial to design forward blur models and efficient optimization algorithms.
The software was used to design forward, reverse and extension primers for the SNPs to be assayed (sequences available on request).
We first design forward observers and derive Linear Matrix Inequalities (LMIs) for the exponential stability of the estimation errors.
Solving the drug war problem -- like so many of the big issues facing humanity - should be about using the best possible research and science to design forward looking policies that are then vetted and embraced by democratic societies.
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