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A deer mouse-specific partial pendrin cDNA sequence was generated, from which Taqman-specific probe and primers were designed for quantification of mRNA equivalents of pendrin gene expression using real-time polymerase chain reaction (PCR).
Since telomeres were constantly subject to recombinant events upon loss of telomerase and thus were likely to relocate or lose the sequence designed for quantification, the maintenance of the URA3 marker in the telomere of type I survivors were confirmed by Southern blot before ChIP analysis (data not shown).
Primer sets for Col3α1 were designed for quantification of DNA within the deleted region.
MSQuant [125] (http://msquant.sourceforge.net/) is a Microsoft.NET software framework designed for quantification studies.
Each TaqMan Low Density Array was designed for quantification of the human PGs.
To determine whether miR-375 was implicated in regulation of selected genes involved in cell cycle, apoptosis, DNA repair, mTOR, or MAPK/ERK pathways, a custom array panel (Roche Applied Science, Manheim, Germany) was designed for quantification of selected gene expression.
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Solid-phase 96-well ELISA plates were used, specifically designed for the quantification of hGH (Quantikine ELISA, R&D Systems) and IFN-α (Verikine Human IFN-alpha ELISA Kit, PBL Assay Science).
FP-XRF units often include analysis modes specifically designed for the quantification of trace elements in soils.
An oligonucleotide chip assay was designed for direct quantification of single strand breaks (SSBs) induced by γ-ray irradiation.
A novel coumarin-derived fluorescent probe, FCP, was designed for Zn2+ quantification based on the photo-induced electron transfer (PET) mechanism.
The novel system was designed for the quantification of the antioxidant efficiency of different potential scavengers of the respective reactive species in an aqueous medium.
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