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Our observations are likely to be useful for the construction of plasmid and viral vectors designed for optimal expression of intronless genes derived from cytoplasmic RNA viruses.
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In the past decade, a variety of codon optimization methods have been used to design genes for optimal expression, which requires selection from a vast number of possible DNA sequences.
Our results will provide an important insight in future studies of protein folding, molecular evolution, and transgene design for optimal expression.
Here, we report on the immunogenicity of a construct harbouring a fusion of the HIV-1 IIIB gag, pol and nef genes (gpn) designed for optimal safety and equimolar expression of the HIV proteins.
All primers were designed for optimal S. cerevisiae codon usage to facilitate expression (see Supplementary Table S1 at http://www.bioscirep.org/bsr/032/bsr0320577add.htm for the primer sequences).
On Tuesday, Stern met with reporters and correctly noted that the timing of this bombshell accusation was designed for optimal media attention during the finals.
- Babies are custom designed for optimal success, attractiveness and other traits deemed most valuable.
Sphere is made with Polartec Power as the pair was designed for optimal performance in frigid conditions.
Cells were visualised using a rapid fluorescence immunostaining method designed for optimal RNA preservation.
The peptides designed for optimal binding were prepared by combining preferred amino acids at each position.
The CC was not designed for optimal mapping resolution.
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