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Here, using two tricistronic vectors designed for expression in mammalian hosts, we present evidence of EMCV IRES activity in prokaryotes.
The nucleotide sequence of 1305 bp ACL racemase gene was designed for expression in Escherichia coli and synthesized in a reaction with 46 oligonucleotides by assembly PCR technique.
We here show that bloodstream forms can be obtained by transient transfection of procyclic forms with a circular plasmid designed for expression of RBP10 from an rRNA promoter.
The ΔPrfc174 mutation was introduced into attenuated Salmonella vaccine strain χ9241 (ΔpabA ΔpabB ΔasdA) followed by introduction of an Asd+ balanced-lethal plasmid to designed for expression of the pneumococcal surface protein PspA.
To elucidate a potential role for this enzyme in diabetic cataract formation, we created a series of transgenic mice designed for expression of human ALR2 (AKR1B1) in epithelial and outer cortical fiber cells of the lens.
From the cloned heavy and light chains of a murine monoclonal antibody (mAbB23) which is specific for human apolipoprotein (apo) B-100 of plasma low-density lipoproteins, a vector was designed for expression of a single-chain antibody (scFv) of mAbB23 in Escherichia coli.
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General expression vectors, designed for intracellular expression or secretion of recombinant proteins in the non-pathogenic Staphylococcus carnosus, were constructed.
This expression vector, designed for protein expression in the methylotrophic yeast Pichia pastoris, contains the Saccharomyces α-factor preprosequence to direct recombinant proteins into the secretory pathway.
The GFP expression vector pHBT, designed for transient expression assays [ 47], was used to construct the ACR11- and ACR12-GFP fusions.
To formally test expression heterochrony, we took advantage of the DTW-S algorithm (Yuan et al., 2011), which has been specifically designed for analyzing expression heterochrony in large-scale gene expression time series.
Finally, we examined the insect transformation functions of two representative members of our large new set of dual piggyBac vectors, one designed for constitutive expression and the other designed for inducible expression of heterologous gene pairs.
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