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In this work, MMPs-degradable gelatin coated mesoporous silica nanoparticles (MSNs@Gel) were designed for endogenous tumor microenvironment-triggered release systems for cancer treatment in vivo.
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A common primer set for all species could be designed for the two endogenous reference genes (Act 5C and fd68A) and two target genes (IM1 and PPO3).
For parasite tag validation studies, reverse transcription and real-time PCR were performed using SYBR Green I Universal PCR MasterMix (PE Applied Biosystems, Foster City, CA, USA) and primers (Additional file 7: Parasite primer sequences for quantitative RT-PCR) were designed for each sequence, including endogenous controls, using Primer express Software (Version 1.5, PE Applied Biosystems).
It is a cyclic heptapeptide and an analogue of endogenous OT designed for longer duration of action [ 12].
A semi-synthetic casein basal diet was designed for measuring the protein and amino acid endogenous flow.
These data indicate that endogenous NAT expression of known genes is potentially detectable by probes designed for the complementary strand of known genes.
Primers were designed for all target genes using Primer Express Software Applied Biosystemss, Foster City, CA), and 18S rRNA was used as an endogenous constitutive control (Additional file 5: Table S3).
The primers are designed without endogenous start and stop codons.
Almost all synthetic pathways for biofuel production are designed to require endogenous metabolites in glycolysis, such as phosphoenolpyruvate, pyruvate, and acetyl-CoA.
The CASP model, first published 1997 by Zantl et al. [12] was designed as an endogenous faecal contamination model.
These microRNA precursor molecules are designed to mimic endogenous microRNAs.
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