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Primers designed for different species are shown in Additional file 3. cDNA was subjected to semiquantitative PCR using the PEDF primers listed below.
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This method has been widely used, with sometimes adjustments from the original strategy, in QTL mapping half-sibs or F2 designs, for different species and different traits, such as growth or immune related traits [e.g. [ 21- 24]].
They were designed for different clients.
Artists often designed for different houses.
Experiments are designed for different purposes.
We have previously developed a technique to study the transcriptomes of heterologous species based on hybridising genomic DNA (gDNA) to a GeneChip array designed for a different species, and subsequently using only those probes with good homology.
For this, we exploited the fact that the SNP array was designed for a different species than the goat (either cattle or sheep).
In addition, unlike many available databases, YMAP is designed to accept genomic data for different species and it can build hapmaps for those genomes if the data for assigning alleles are available.
The simulation model is designed to be flexible so it can be used for different species and fisheries.
Such differences may be useful for the design of PFTase inhibitors that are selective for different species.
"I started searching around for different species to grow and different ways of growing them".
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