Sentence examples for designed for cloning from inspiring English sources

After sequencing the PCR fragment to confirm the presence of IMNV, additional primers were designed for cloning and sequencing the full 7.5 kb IMNV genome.

Primers were designed for cloning the cDNA encoding human MIF.

The DNA cassette was initially designed for cloning into the retroviral expression vector pSuper (OligoEngine).

Because of high similarity in the gloverin family, specific forward primers for Bmglv2 and Bmglv3 were designed, while a degenerate forward primer was designed for cloning Bmglv4, and common reverse primer was used for Bmglv 2 3 (Table S1).

The third vector, pARS-ALF, was designed for cloning human alphoid DNA arrays.

The siRNAs for HPV16 E6 and E7 were designed for cloning into the pSilencer 1.0-U6 plasmid.

All singletons and one randomly chosen clone from each contig were fully sequenced in both directions, with internal sequencing primers designed for clones longer than 1 kb.

The DNA sequence of E. coli strain K-12 was obtained from the JCVI/CMR Genome Database (http://cmr.jcvi.org/cgi-bin/CMR/CmrHomePage.cgi) and primers were designed for clones which were cloned by Gateway® recombination, using the primer design tool (http://tools.bio.anl.gov/bioJAVA/jsp/ExpressPrimerTool/).bio.anl.gov/bioJAVA/jsp/ExpressPrimerTool/

Based on the amino acid sequences of two peptides generated by enzymatic digestion, degenerate primers were designed for cDNA cloning.

Specific primers of all candidates were designed for sequencing, cloning and to confirm the homology with Drosophila and Tribolium (Supplementary file 1).

ZK designed strategies for cloning MA genes, assisted in the manual re-annotation, and participated in drafting the manuscript.