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Additionally, the hopper outlet should be designed close to the centerline of the blast furnace to reduce the particle size segregation.
The primers for COBRA or pyrosequencing analysis were designed close to the promoter region in each case (Figure 1). Figure 2 shows examples of the COBRA and pyrosequencing analysis.
The immunoprecipitated DNA was amplified by real-time PCR using Fast SYBR Green Master Mix Applied Biosystemss, Warrington,UK) Two pairs of primers were designed for DBN1, SETMAR and HIG2 with one primer designed close to transcription start site (TSS) and one distal (∼2 kb) to the TSS (DT).
Therefore, sequencing primers designed close to these regions were used in reactions employing alternate chemistries.
About 800 oligonucleotide probes were designed close to the ends of the fragments.
The probe was designed close to the 3'-UTR of the gene and its length was 202 bp.
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"It is just possible that the programs designed by advertisers to draw young eyeballs would be somewhat different if they were designed closer to the community," he said.
Experiments were designed closer to examine the subcellular expression of SUR1 subunits relative to Kir6 pore-forming subunits (Fig 8).
To achieve a higher success rate, another set of primers was also were designed closer to the SSR motifs in order to capture short length reads included in contigs.
The test designs closest to the conditions in the stream mesocosms reflected best the results in mesocosms study on basis of LOEC values.
The results already show two designs close to competitiveness.
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