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Briefly, MET1 specific probes ranging from 60 to 90 nucleotides were designed at a density of ~1.10 probes/Mb of sequence by Roche-Nimblegen from one Triticum aestivum public EST (accession number TA8302), one private EST (GPIC:S 720428) and from the two coding sequences from Brachypodium distachyon (Accession numbers [Phytozome: Bradi1g05380 and Bradi1g55290]).
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Roche Nimblegen Systems (Madison, WI, USA) designed an oligonucleotide array at a density of 385K.
The experimental design used a latin square with 4 replications, each containing 20 plants at a density of 3 plants/m.
The experimental design involved cells being grown for seven days in six-well tissue culture plates at a density of 12.10 cells per ml.
Cells were seeded at a density of 5000 10,000 cells/well.
They spread across the country at a density of 12 per acre and home ownership hit 32percentnt in 1938.
at a density of 16 000 cells/cm.
at a density of 300 000 cells/dish.
The localization density map of a subunit was contoured at a density of 0.15.
But such a density of information is at odds with the prevailing orthodoxy in graphic communication: economy.
Comparative test results showed the proposed design surpasses performance of both parallel and interdigitated flow field design at operation current density of 0.7 A cm−2 or higher.
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