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The designed assay is rapid, convenient, flexible, and reliable.
The newly designed assay has a limit of detection of 10 copies per reaction.
The newly designed assay was successfully applied for the detection and quantification of HM in horses in Germany.
The designed assay was shown to be a valuable epidemiological tool for the purpose of snail infection monitoring.
Genotyping of SCN1A isS5-91G>A is done by Taqman custom designed assay; in a real time7500HT System.
Using a specifically designed assay and ligand-based NMR screening of a fragment library, we identified a thioenylaminopyrazole compound with a dissociation constant of ∼500 μM.
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Carefully designed assays of antimicrobial activity may also be useful.
We designed assays for 3 of these SNPs.
Three of the designed assays failed in the qRT-PCR validations.
PMcK designed assays, performed sequencing and pyrosequencing, and prepared the manuscript.
Newly designed assays must always be validated using methods that can discriminate between individual alleles.
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