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We designed an "overlap" method that incorporated repeat masking data generated using the RepeatMasker platform [ 45] to identify genomic positions of the same 7080 annotated Hexapoda TEs sequences from RepBase [ 42].
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Model 2 was designed with an overlap distance between the adjacent blades, a = 25 mm but having the same blade diameter and height as model 1, and was fabricated with the same number of blades 120° apart.
To overcome this limitation, we designed an Nv eve morpholino overlapping the translation start site, as well as one directed at the exon intron junction in the homeobox.
The ENGAGE AF-TIMI 48 investigators designed a 14-day overlap protocol, and the choice of OAC at the end of the trial (VKA or an NOAC) was made by the physician and patient.
In this study, we designed a set of overlapping peptides based on the RL42 Gag sequence, and we performed ELISPOT assays of samples from nine B′ clade HIV-1-infected HLA-A∗30-B∗13-C∗06-positive slow progressors from the paid plasma donor cohort in the Henan province.
In detail, we designed a pair of overlapping primers (KCBP-T982N-R and KCBP-T982N-F) with the desired nucleotide changes at the target site, and amplified the front half with primers of KCBP2X-F and KCBP-T982N-R, and that latter half with primers of KCBP-T982N-F and GA-KCBP-R, then the two fragments were linked together by Fusion PCR using primers of KCBP2X-F and GA-KCBP-R.
The CLDN11 RT-PCR amplicon was designed to overlap an intron-exon boundary in order to exclude genomic DNA (gDNA) amplification.
The study period was designed to overlap a second day (but not to exceed 24 hours) to ensure that data acquisition in each unit included at least one day shift and one night shift.
Primer 7120RP is designed to have an overlap with the donor plasmid to facilitate cloning Transformation of R. oryzae M16 spores was achieved by particle bombardment.
The primers were designed to provide an overlap with the data obtained from TSPs1 3, and hence confirm that the sequence retrieved was an extension of the previous mRNA and not a new variant with a truncated 3′ end.
For CACYBP, it was not possible to design a pyrosequencing assay overlapping the Illumina CpG site.
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