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Of the inbred strains that have been created, the two still used with any frequency are, following Sewall Wright's designations, "Strain 2" and "Strain 13".
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Therefore we have provided the wildtype N2 grown in CeMM for three years with a new name under our registered lab designation, strain CC1.
MMEs and candidate (c MMEs were identified based on the criteria given in [26], [70], and the phage designations for strains α14, FAM18, MC58 and Z2491 were taken from [12], [14], [27], [64], [65], [71].
Briefly, a culture of Staphylococcus aureus ATCC #6538 (designation FDA 209 strain) was diluted in 1.5% peptone water to a concentration to yield challenge levels of 2200±500 colony forming units (CFU) per test sample.
For diploid genomes, each transcript identifier corresponds to two allelic sequences that are further differentiated with the L/R chromosome designation and their A H founder strain designation (e.g., ENSMUST00000000001FL and ENSMUST00000000001HR).
†International MRSA strain designations, except for AK3, which is a New Zealand designation for a community-associated MRSA strain common in New Zealand.
The names of restriction enzymes are derived from the genus, species, and strain designations of the bacteria that produce them; for example, the enzyme EcoRI is produced by Escherichia coli strain RY13.
Note that species and strain designations are as in [26], except Shewanella putrefaciens CN32, which was originally described in [27].
Therefore, the use of strain designations is still appropriate.
Isolates were given specific strain designations based on at least 80% similarity.
PFGE is highly reliable and a gold standard for strain designations.
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