Sentence examples for designated vector from inspiring English sources

Stable cell lines were established by transfecting Vector DNA, wild-type Aurora-A or kinase-inactivated Aurora-A into 7-4 cells, which was derived from NIH/3T3 cells harboring the inducible Ha- rasV12 oncogene [ 23], designated Vector, WT and KD cell line, respectively.

All constructs were cloned by PCR into the designated vectors, and oligo sequences are available upon request.

The modifications to the vector were again confirmed by sequencing, and we designated the designed vector as pAAV-LIC.

HEMs and HEKs transfected with the empty lentiviral vector (designated as VECTOR) were used as controls.

Tumors with vector-expressing cells (designated as vector tumors) were very sensitive to 5-FU treatment and failed to grow during drug treatment, whereas tumors with miR-587-expressing cells (designated as miR-587 tumors) were relatively resistant to 5-FU treatment and continued to grow at a steady rate.

Transgenic tobacco transformed with the empty vector pBI121 was used as negative control (designated as "vector control") in this study.

To obtain relative values, the following arithmetic formula was used: 2-ΔΔCT, where ΔCT = difference between the threshold cycles of the target and an endogenous reference (actin), and -ΔΔCT = difference between ΔCT of the target sample and a designated calibrator (vector control).

The control vectors were designated as Empty vector or pcMV-control.

We employed PC3 cells that lack the functional AR to investigate a cooperative role between AR and cystatin C. PC3 cells were co-transfected with AR expressing vector along with cystatin C siRNA vector designated as "siCysC, AR" or with a control vector designated as "sictr, AR".

This vector, designated pBEV, was specifically designed to exploit the advantages that both hosts would provide.

The resulting vector was designated the pBE-CD vector.