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Concentrations of total actin, including F-actin and G-actin (actin monomer), and total PKMζ mRNA (mRNA in and out of the translational machinery) are both assumed to be constant and designated values of one since neither actin or PKMζ mRNA has been shown to increase or decrease upon LTP induction.
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The representation in question is built on a single initial value which serves then as the designated value of the resulting valuation system.
The algorithm was run using the admixture model initially, with 20 replicates of each designated value of K (K = 1 to K = 10).
The set D of designated values is of central importance for the notion of a valuation system.
For many-valued logics, validity is accounted for in terms of preservation of designated value, where designated values can be thought of as ways of being true, while non-designated values can be thought of as ways of being false.
In the literature on many-valued logic it is sometimes proposed to consider a set of antidesignated values which not obligatorily constitute the complement of the set of designated values (see, e.g., (Rescher 1969), (Gottwald 2001)).
If we define a consequence relation in terms of preservation of these designated values, then we have the paraconsistent logic LP of Priest 1979.
The logical values are thus represented by a bi-partition of the set of algebraic values into a set of designated values (truth) and its complement (falsity).
Malinowski (1990) proved that for every structural q-consequence relation, there exists a characterizing class of q-matrices, matrices which in addition to a non-empty subset D+ of designated values comprise a disjoint non-empty subset D- of antidesignated values.
Whereas the algebraic values that are not designated are already given with the set of designated values D as its set-theoretical complement, the treatment of true and false as values that are independent of each other leads to distinguishing between the set D+ of designated values and the set D− of anti-designated values.
There are then two non-trivial choices: let the set of designated values be {true} or {true, indeterminate}.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com