For this, the 'Long Trunk' sequences that were designated to the other parental species were excluded.
Most sequences matched 16S rRNA sequences in the database with E values of 0 and were designated to be the same sequence.
Due to High Resolution Melting Analyses (A segregation technique using Melting point of DNA) the Rotor Gene 6000 Corbettt) 20 samples were designated to be sequenced only in forward direction.
Multilocus sequence typing (MLST) was performed on all E. coli isolates by using 7 housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA) to determine the corresponding sequence type (ST) and to designate the sequence type complex (STC) by using the MLST Databases at the Environmental Research Institute, University College Cork website (http://mlst.ucc.ie/mlst/dbs/Ecoli).ucc.ie/mlst/dbs/Ecoli
Historically, the term MLE was used to designate any sequence showing sequence similarities with the original mariner elements (peach and Mos1 copies), including consensus sequences derived from independent, closely related clones identified in the same species [ 6, 7].
However, we do not extend this terminology to designate the longest sequence to literally represent all the clustered sequences.
The reactivity of pool F was found to be located in the single peptide designated F9.1 having the sequence GFAEINPIA.
For convenience, we designated the sequence type for each individual of E. prinoides var.
Here, "motif" and "segment" are used to designate the five-member sequence (EPIYA) and the short sequences around the EPIYA motif, respectively (Figure 1).
The first nucleotide of GmPRP2 cDNA was designated as +1 to orient the sequence numbers.
On the basis of the nucleotide profile observed, the 3′ UTR plus 100 nt sequence downstream of the poly(A) site was divided into six signal element regions, designated regions I VI, to identify the sequence elements for 3′-end-processing (Fig. 3C).
