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The mechanical properties of the retrieved diaphragmatic scaffolds were similar to those of normal diaphragm at the designated time points.
At the designated time points, groups of mice (n = 4 5) were humanely sacrificed by over-inhalation of isoflurane.
At the designated time points, the medium was discarded and the cells were rinsed twice with cold PBS.
We found that bone mass consistently decreased and the bone microarchitectures continuously deteriorated in male ARKO mice at designated time points.
Efficacy: Difference in remission (YMRS (Young et al. 1978) score of ≤12 or equivalent) between lithium and comparatively treated patients at designated time points.
After irradiation, chromosomal aberrations of cells were analyzed at designated time points using G2 phase premature chromosome condensation (G2-PCC) coupled with Giemsa staining and with multiplex fluorescent in situ hybridization (mFISH).
Energy metabolism was evaluated at designated time points throughout the study by performing morning oral glucose tolerance tests and measuring levels of glucose, insulin, glucagon, leptin, ghrelin, adiponectin, resistin, and brain-derived neurotrophic factor (BDNF).
At designated time points, mice were euthanized by CO2 asphyxia.
At designated time points after inoculation, mice were euthanized by cervical dislocation under anesthesia.
Cells were fixed at designated time points with 4% paraformaldehyde for 10 minutes.
At designated time points, cells were harvested and fixed in 1% paraformaldehyde.
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