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After the designated time point, the whole tree was felled and two types of samples were taken: (1) wounded area (S1) and (2) 5 cm below S1 (S1).
Peptides at concentrations ranging from 10−5 to 10−12 M were added at designated time point and calcium level was traced in a real-time manner using the Fluoview software (Olympus).
Tubes were agitated for 0, 60, 120, or 180 min. To determine the number of viable bacteria after exposure to serum, an aliquot of each bacterial suspension was removed at the designated time point, diluted 10-fold by the addition of Mueller-Hinton broth, plated on Mueller-Hinton agar, and assayed as described below.
Cells were collected and counted at each designated time point.
The baseline step (autumn/winter 2009) is designated time point 'and and the event step (autumn/winter 2009) is designated time point 'B'.
To visualize the antitumor effect, bioluminescence was performed at a designated time point.
Similar(38)
The mechanical properties of the retrieved diaphragmatic scaffolds were similar to those of normal diaphragm at the designated time points.
At the designated time points, groups of mice (n = 4 5) were humanely sacrificed by over-inhalation of isoflurane.
At the designated time points, the medium was discarded and the cells were rinsed twice with cold PBS.
We found that bone mass consistently decreased and the bone microarchitectures continuously deteriorated in male ARKO mice at designated time points.
Efficacy: Difference in remission (YMRS (Young et al. 1978) score of ≤12 or equivalent) between lithium and comparatively treated patients at designated time points.
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