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In brief, suspension cells generated from green, véraison and ripe berries (designated stages I-III) in their log and early stationary phases were separated from the growth medium using Mira cloth and ground in liquid nitrogen.
After the joint assessment, all participants were instructed to adjust, if necessary, their originally designated stages.
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The lapses in discipline led firefighters to rush to the scene without checking in with commanders at designated staging areas.
These time points corresponded to green, véraison and ripe berries and the suspensions were designated Stage I to III respectively.
To label filamentous actin (F-actin), zebrafish embryos at designated stage were fixed in 4% paraformaldehyde in PBS overnight at 4°C, washed in PBS and manually dechorionated.
Fold change for each gene at the designated stage is indicated.
Tumor stage was determined at initial exploration, with 86 patients (27.2%) designated Stage I, 98 (31.0%) Stage II, 80 (25.3%) Stage III, 41 (13.0%) Stage IV, and 11 (3.5%) Stage V.
Because of budget concerns, seven laboratories began testing approximately 1 year before the others; these tests were designated stage 1.
Cells at the designated stage of induction of differentiation were washed twice with PBS and lysed by brief sonication.
Any metastatic disease is automatically designated Stage IV disease and with few exceptions is considered surgically unresectable.
This subgroup, designated stage 4s (s for special), has been recognized as a distinct clinical entity in all subsequent classifications of NB [ 2].
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CEO of Professional Science Editing for Scientists @ prosciediting.com