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This isolate was designated sequence type ST404 [ 34].
This increase is largely the result of the widespread emergence of a single disseminated E. coli clonal group, designated sequence type (ST) 131 according to multilocus sequence typing (MLST) (1, 2 ).
The total synthesis of these substances in quantity has not only allowed a broad array of biological studies to be carried out but also has provided the chemical basis for the site-specific incorporation of AA metabolites into oligomeric DNA of any designated sequence (unpublished material).
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For cloning of level 0 modules, the designated sequences are PCR-amplified, adding the respective fusion site and a BpiI recognition site as part of the primers used for amplification, and cloned via a BpiI Golden Gate cloning reaction.
Among these, 16 were newly designated sequences.
Over 16,000 rbcL sequences were found to be orthologous to the designated sequences sampled throughout flowering plants.
Here, we determine orthologous sequences using designated sequences representing the breadth of variation observed in the gene region of interest across the clade of interest.
We BLAST all of the potential sequences from the database against these designated sequences and other potential sequences that are determined to match with a certain threshold (i.e. according to both coverage and identity).
Much attention has been devoted to this part of the genome, especially since 1980, when the term "selfish" DNA was introduced to designate sequences that propagate themselves within a genome, without contributing to the development of the organism [4], [5].
In the following sections the term ortholog will be used to designate sequences that are derived from a single ancestral gene in the last common ancestor of the species that are being compared [ 35].
The process, designated "tagged sequence mutagenesis", involves sequencing short segments of DNA isolated from each mutation to identify genes disrupted by the targeting vector.
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