Our classification methodology allows morphological class populations to be designated with minimal (and measurable) ambiguity simplifying the study of SLC degradation at different target regions.
Minimizers, expressions designating a minimal amount or referring to an end point of a scale, are frequently employed for emphatic functions (Fauconnier 1975; Haspelmath 1997; Horn 1989; Israel 2011).
Proof If we consider a subtree with the node k as a root then σ k (i ) designates the minimal cost, given that node k has a label i : (A.1) S (G ) = min i σ N (i ) = min i [ φ (π (r t ), π (l ) ) + φ (π (r t ), π (r ) ) + σ l (π (l ) ) + σ r (π (r ) ) ]. Proof of the subclaims (1)–(3) is trivial.
This is designated as the minimal transient discrete cue (MTDC) protocol.
This has previously been designated as the minimal transient discrete cue (MTDC) protocol [ 11- 16].
The University of Washington Human Subjects Division reviewed the protocol and designated this study Minimal Risk and granted a waiver of consent due to the nature of the retrospective review and inability to contact subjects that were included in the database (HSD Study #43072).
To ensure a maximum reproducibility with the Y2H interactions, sequence data from the Y2H screen was used to define the smallest cDNA fragment identified among all clones obtained for each interaction in the Y2H screen (designated as the minimal interacting region, or MIR).
Strains were designated resistant if their minimal inhibitory concentration was ≥ 4 μg/mL.
A DNA fragment selected by CAST containing two Fli-1 binding sites was cloned into the pGL4.28 luciferase reporter (Promega, Madison, WI, USA) containing a minimal promoter, designated Fli-Lu.
To this end two reporter gene constructs were generated that contained either a cluster of three proximal (−669/−218) or three distal (−1214/−669) SBE's upstream of a minimal promoter (constructs designated pTAZ-BRE/P and pTAZ-BRE/D, respectively).
Markers included in this minimal combination are designated as irregular (homoplastic) markers, while the rest represents the maximal combination of compatible characters.
